Microinjection

In this section:

Ficoll, 2.5%

2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)

3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)

Ficoll, 2.5%

2.5 g ficoll/100ml DI water (usually make 500 ml)

Autoclave and store at RT.

Use in place of water in dish to stabilize embryos

2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)

Dissolve 2.5 g/100ml of Ficoll in 1/3X MR and autoclave(Takes long time to dissolve.)

Store at RT.

3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)

Dissolve 3 g/100ml of Ficoll in 1/9X MR and autoclave(Takes long time to dissolve.)

Store at RT