Dejellying solution (Zorn lab)
Dejellying solution:
2% Cysteine-hydrochloride, pH 8 in 0.1xMBS, to remove the jelly coat from fertilised Xenopus eggs and embryos before they can be manipulated.
| Volume of 0.1xMBS | Cysteine | 5N NaOH |
|---|---|---|
| 50 mls | 1g | to pH 8.0 |
| 100 mls | 2g | to pH 8.0 |
| 200 mls | 4g | to pH 8.0 |
Dissolve the Cysteine hydrochloride (or L-Cysteine hydrochloride-1-hydrate) in ~3/4 the final volume of 0.1xMBS.
Adjust to ~ pH 8 with 5N NaOH.
Check that the pH is correct and then adjust the final volume with 0.1xMBS.
This solution will only last one day.