Monoclonal 4A6 - pronephros

Generated and characterized by Elizabeth Jones [1]. References: Dev Bio 171:531- [2]

Antibody name: 4A6

Raised in: mouse, monoclonal

Subtype: IgG

Antigen: dissected pronephroi. Specific target unknown.

Developmental Window: First detects target at stage 39, continues until latest stages tested.

Stains: distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.

Fixatives: MEMFA

Sensitive to bleaching: No

Immunoprecipitation/Western blotting: Works with both

Available as: cell supernatent, 3 x

Available from; Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.

Optimized conditions: Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.

Other species: Xenopus only

References: Dev Bio 171:531- [3]