12/101 – somites (monoclonal)

Antigen: homogenized tissue

Epitope:

Host Species/Isotype: mouse monoclonal IgG

Antigen Species: newt

Applications: IF, WB

Antibody Name: 12/101

Supplier: Developmental Studies Hybridoma Bank

Comments: WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)

Contributor: Generated and characterized by Kintner [1] and Brockes (1985) [2]

applications abbreviations: WB - Western blotting, IP - immunoprecipitation, IF - immunofluorescence, IHC - immunohistochemistry, EMSA - electromobility shift assay, ChIP - chromatin immunoprecipitation, FCM - flow cytometry

Immunofluorescence using 12/101

Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.