12/101 - somites, skeletal muscle marker: Difference between revisions

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'''Antigen:''' homogenized tissue
'''Antigen:''' homogenized tissue
'''Epitope:'''
'''Xenbase Gene Page:'''
'''Host Species/Isotype:''' mouse monoclonal IgG
'''Epitope:'''
'''Antigen Species:''' newt
'''Host Species/Isotype:''' mouse monoclonal IgG
'''Applications:''' IF, WB
'''Antigen Species:''' newt
'''Antibody Name:''' 12/101
'''Applications:''' IF, WB
'''Supplier:''' Developmental Studies Hybridoma Bank
'''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/skeletal-muscle-marker 12/101]
'''Comments:''' WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)
'''Supplier:''' Developmental Studies Hybridoma Bank
'''Contributor:''' Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901]
'''Comments:''' WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)
'''Contributor:''' Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.do?method=display&articleId=40901]
'''Additional Information:'''
'''Reference(s):'''


applications abbreviations:
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry


[[File:12101.jpg]]
WB - Western blotting,
Immunofluorescence using 12/101
 
IP - immunoprecipitation,
[[File:12101+ATPase.jpg]]
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry
[[File:12101.jpg]]
Immunofluorescence using 12/101
[[File:12101+ATPase.jpg]]
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.

Latest revision as of 12:58, 8 March 2011

Antigen: homogenized tissue

Xenbase Gene Page:

Epitope:

Host Species/Isotype: mouse monoclonal IgG

Antigen Species: newt

Applications: IF, WB

Antibody Name/Catalog Number: 12/101

Supplier: Developmental Studies Hybridoma Bank

Comments: WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)

Contributor: Generated and characterized by Kintner [1] and Brockes (1985) [2]

Additional Information:

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry


Immunofluorescence using 12/101

Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.

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