12/101 - somites, skeletal muscle marker

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Antigen: homogenized tissue

Xenbase Gene Page:


Host Species/Isotype: mouse monoclonal IgG

Antigen Species: newt

Applications: IF, WB

Antibody Name/Catalog Number: 12/101

Supplier: Developmental Studies Hybridoma Bank

Comments: WB - 102 kDa target, IF - not sensitive to bleaching, other species reactive (chick, mouse, newt, rabbit)

Contributor: Generated and characterized by Kintner [1] and Brockes (1985) [2]

Additional Information:


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

Immunofluorescence using 12/101

Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.