Alpha-tubulin - tuba4b - microtubules: Difference between revisions

Latest revision as of 10:01, 9 March 2011

Antigen: microtubules from chick embryo brain

Xenbase Gene Page: tuba4b

Epitope:

Host Species/Isotype: mouse IgG

Antigen Species: chicken

Applications: IF, WB

Antibody Name/Catalog Number: DM1A (cat#T9026)

Supplier: Sigma

Comments: IF: MEMFA 1hr @ RT, post-fix Dent's use at 1:300, sensitive to bleaching, functions post in situ, WB: use at 1:1000 to 1:10,000

Contributor: Wallingford Lab, Zorn Lab

Additional Information: other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

@@ Line 1: / Line 1: @@
-'''Antigen:'''			microtubules from chick embryo brain
+'''Antigen:'''		microtubules from chick embryo brain
-'''Epitope:'''
-'''Host Species/Isotype:'''			mouse IgG
-'''Antigen Species:'''			chicken
-'''Applications:'''
-'''Antibody Name:'''			DM1A
-'''Supplier:'''			Sigma (cat#T9026)
-'''Comments:'''			fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
-'''Contributor:'''			Wallingford Lab
-applications abbreviations:
+'''Xenbase Gene Page:'''		[http://www.xenbase.org/gene/showgene.do?method=display&geneId=489926 tuba4b]
-WB - Western blotting,
-IP - immunoprecipitation,
+'''Epitope:'''
-IF - immunofluorescence,
-IHC - immunohistochemistry,
-EMSA - electromobility shift assay,
-ChIP - chromatin immunoprecipitation,
-FCM - flow cytometry
-Additional Comments:
+'''Host Species/Isotype:'''		mouse IgG
-'''Available as:''' Ascites
-'''Optimized conditions:''' We (Wallingford lab) dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room temp. for four hours.
+'''Antigen Species:'''		chicken
+'''Applications:'''		IF, WB
-'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
+'''Antibody Name/Catalog Number:'''		DM1A [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=T6199|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC (cat#T9026)]
+'''Supplier:'''		Sigma
+'''Comments:'''		IF: MEMFA 1hr @ RT, post-fix Dent's use at 1:300, sensitive to bleaching, functions post in situ, WB: use at 1:1000 to 1:10,000
+'''Contributor:'''		Wallingford Lab, Zorn Lab
+'''Additional Information:'''		other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
+'''Reference(s):'''
+applications abbreviations:
+WB - Western blotting,
+IP - immunoprecipitation,
+IF - immunofluorescence,
+IHC - immunohistochemistry,
+EMSA - electromobility shift assay,
+ChIP - chromatin immunoprecipitation,
+FCM - flow cytometry

Alpha-tubulin - tuba4b - microtubules: Difference between revisions

Latest revision as of 10:01, 9 March 2011

Navigation menu