Alpha-tubulin - tuba4b - microtubules: Difference between revisions

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'''Antigen:''' microtubules from chick embryo brain
'''Antigen:''' microtubules from chick embryo brain
 
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=489926 tuba4b]
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=489926 tuba4b]
'''Epitope:'''
'''Epitope:'''
 
'''Host Species/Isotype:''' mouse IgG
'''Host Species/Isotype:''' mouse IgG
 
'''Antigen Species:''' chicken
'''Antigen Species:''' chicken
'''Applications:'''
'''Applications:''' IF, WB
 
'''Antibody Name/Catalog Number:''' DM1A [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=T6199|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC (cat#T9026)]
'''Antibody Name/Catalog Number:''' DM1A [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=T6199|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC (cat#T9026)]
 
'''Supplier:''' Sigma
'''Supplier:''' Sigma
 
'''Comments:''' fixatives: MEMFA or Dent's, sensitive to bleaching, functions post in situ
'''Comments:''' IF: MEMFA 1hr @ RT, post-fix Dent's use at 1:300, sensitive to bleaching, functions post in situ, WB: use at 1:1000 to 1:10,000
 
'''Contributor:''' Wallingford Lab
'''Contributor:''' Wallingford Lab, Zorn Lab
 
'''Additional Information:''' other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
'''Additional Information:''' other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
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IP - immunoprecipitation,
IP - immunoprecipitation,
 
IF - immunofluorescence,
IF - immunofluorescence,
 
IHC - immunohistochemistry,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry
FCM - flow cytometry

Latest revision as of 10:01, 9 March 2011

Antigen: microtubules from chick embryo brain

Xenbase Gene Page: tuba4b

Epitope:

Host Species/Isotype: mouse IgG

Antigen Species: chicken

Applications: IF, WB

Antibody Name/Catalog Number: DM1A (cat#T9026)

Supplier: Sigma

Comments: IF: MEMFA 1hr @ RT, post-fix Dent's use at 1:300, sensitive to bleaching, functions post in situ, WB: use at 1:1000 to 1:10,000

Contributor: Wallingford Lab, Zorn Lab

Additional Information: other species: yeast, human, chicken, rat, amphibian, fungi, bovine, mouse

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry