Anti-FLAG M2 monoclonal antibody: Difference between revisions
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Revision as of 09:04, 8 March 2011
Antigen: FLAG epitope
Epitope: aa DYKDDDDK
Host Species/Isotype: mouse monoclonal
Antigen Species: synthetic peptide
Applications: IP, IF, WB
Antibody Name: F3165 [1]
Supplier: Sigma
Comments: IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000
Contributor: Zorn Lab and V Ponferrada
Additional Information: IF: MEMFA, 10% TCA fixatives work
Reference(s):
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry