Anti-FLAG M2 monoclonal antibody

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Antigen: FLAG epitope

Xenbase Gene Page:

Epitope: aa DYKDDDDK

Host Species/Isotype: mouse monoclonal

Antigen Species: synthetic peptide

Applications: IP, IF, WB

Antibody Name/Catalog Number: F3165

Supplier: Sigma

Comments: IP: use 2 ug, IF: use at 1:1000, WB use at 1:5000 to 1:10,000

Contributor: Zorn Lab and V Ponferrada

Additional Information: IF: MEMFA, 10% TCA fixatives work


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry