Antibody template: Difference between revisions

Revision as of 11:27, 16 June 2010

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Antibody name:

Raised in:

Subtype:

Antigen:

Fixatives:

Sensitive to bleaching:

Functions post in situ?:

Immunoprecipitation/Western blotting:

Available as:

Available from:

Optimized conditions:

Other species:

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-Alpha-tubulin antibody for labeling microtubules
+To use this template, click
-'''Antibody name:''' DM1A
+'''Antibody name:'''
-'''Raised in:''' Mouse
+'''Raised in:'''
-'''Subtype:''' IgG
+'''Subtype:'''
-'''Antigen:''' Microtubules from chick embryo brain
+'''Antigen:'''
-'''Fixatives:''' Works well in MEMFA or Dent's
+'''Fixatives:'''
-'''Sensitive to bleaching:''' Yes
+'''Sensitive to bleaching:'''
-'''Functions post in situ?:''' Yes
+'''Functions post in situ?:'''
 '''Immunoprecipitation/Western blotting:'''
-'''Available as:''' Ascites
+'''Available as:'''
-'''Available from:''' Sigma (cat#T9026)
+'''Available from:'''
-'''Optimized conditions:''' We dilute 1:1000 and incubate in primary antibody overnight at 4 degrees or room tempp for four hours.
+'''Optimized conditions:'''
-'''Other species:''' yeast, human, chicken, rat, amphibian, fungi, bovine, mouse
+'''Other species:'''