C-cadherin - cdh3: Difference between revisions

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'''Antigen:''' C-cadherin
'''Antigen:''' C-cadherin
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=482276 cdh3]
'''Epitope:''' C-cadherin extracellular domain fused to GST
'''Epitope:''' C-cadherin extracellular domain fused to GST
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'''Applications:''' IF
'''Applications:''' IF
'''Antibody Name:''' 6B6 [http://dshb.biology.uiowa.edu/cadherin-C-]
'''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/cadherin-C- 6B6]
'''Supplier:''' Developmental Studies Hybridoma Bank
'''Supplier:''' Developmental Studies Hybridoma Bank
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'''Comments:''' IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300
'''Comments:''' IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300
'''Contributor:''' Zorn Lab and V. Ponferrada
'''Contributor:''' Zorn Lab and V Ponferrada
'''Additional Information:''' MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work
'''Additional Information:''' MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work
'''Reference(s):'''
'''Reference(s):'''

Latest revision as of 15:32, 8 March 2011

Antigen: C-cadherin

Xenbase Gene Page: cdh3

Epitope: C-cadherin extracellular domain fused to GST

Host Species/Isotype: mouse monoclonal

Antigen Species: xenopus

Applications: IF

Antibody Name/Catalog Number: 6B6

Supplier: Developmental Studies Hybridoma Bank

Comments: IF: directly fix in Dent's 2 hrs @ RT then to 100% methanol use at 1:300

Contributor: Zorn Lab and V Ponferrada

Additional Information: MEMFA, 10% TCA, and 4% PFA in 1xPBS fixatives also work

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry