C-myc - myc - from roche: Difference between revisions

Revision as of 15:51, 8 March 2011

Antigen: E-cadherin

Xenbase Gene Page: cdh1

Epitope: 140 kDa xenopus E-cadherin

Host Species/Isotype: mouse monoclonal IgG2b

Antigen Species: xenopus

Applications: IF

Antibody Name/Catalog Number: 5D3

Supplier: Developmental Systems Hybridoma Bank

Comments: IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300

Contributor: Zorn Lab and V Ponferrada

Additional Information: MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

@@ Line 1: / Line 1: @@
-'''Antigen:'''		c-myc
+'''Antigen:'''		E-cadherin
-'''Xenbase Gene Page:'''		[http://www.xenbase.org/gene/showgene.do?method=display&geneId=6052813 myc]
+'''Xenbase Gene Page:'''		[http://www.xenbase.org/gene/showgene.do?method=display&geneId=12531593 cdh1]
-'''Epitope:'''		aa 408-439 of human c-myc
+'''Epitope:'''		140 kDa xenopus E-cadherin
-'''Host Species/Isotype:'''		mouse monoclonal
+'''Host Species/Isotype:'''		mouse monoclonal IgG2b
-'''Antigen Species:'''		human
+'''Antigen Species:'''		xenopus
 '''Applications:'''		IF
-'''Antibody Name/Catalog Number:'''		[https://www.roche-applied-science.com/servlet/RCProductDisplay?storeId=10202&catalogId=10202&langId=-1&countryId=us&forCountryId=us&productId=3.7.2.12.10.1 anti-c-myc (clone 9E10) 1 667 203]
+'''Antibody Name/Catalog Number:'''		[http://dshb.biology.uiowa.edu/cadherin-E- 5D3]
-'''Supplier:'''		Roche
+'''Supplier:'''		Developmental Systems Hybridoma Bank
-'''Comments:'''		IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, 0.5 ug/ml use at 1:500 to 1:1000
+'''Comments:'''		IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300
-'''Contributor:'''		Zorn Lab
+'''Contributor:'''		Zorn Lab and V Ponferrada
-'''Additional Information:'''
+'''Additional Information:'''		MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work
 '''Reference(s):'''