High-salt solution for egg laying (Zorn lab/Ron Laskey): Difference between revisions
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___________________(1x solution)___________________________amounts to make 8X | ___________________(1x solution)___________________________amounts to make 8X stock_________________ | ||
_______________________________________________2 litres___________4 litres____________8 liters_________ | |||
NaCl_________________110 mM___________________102.85g___________205.71g___________308. | NaCl_________________110 mM___________________102.85g___________205.71g___________308.55g________ | ||
Tris base______________15 mM____________________29.075g___________58.15g____________87. | Tris base______________15 mM____________________29.075g___________58.15g____________87.23g________ | ||
KCl____________________2 mM____________________2.385g_____________4.77g____________7. | KCl____________________2 mM____________________2.385g_____________4.77g____________7.2g________ | ||
Na2HPO4 (anhydrous)___0.5 mM____________________1.36g______________2.72g____________4. | Na2HPO4 (anhydrous)___0.5 mM____________________1.36g______________2.72g____________4.1g________ | ||
NaHCO3________________2 mM____________________2.69g______________5.38g____________8. | NaHCO3________________2 mM____________________2.69g______________5.38g____________8.07g________ | ||
Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres) | :Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres) | ||
MgSO4.7H2O____________1mM____________________7.89g______________3.945g___________11. | MgSO4.7H2O____________1mM____________________7.89g______________3.945g___________11.84g________ | ||
Make 8X stock, dilute to 1x solution in dH2O just before use. Maximum recommended storage: 8 weeks at 4oC. | Make 8X stock, dilute to 1x solution in dH2O just before use. Maximum recommended storage: 8 weeks at 4oC. | ||
Revision as of 09:59, 23 November 2011
Protocol submitted by VGP
RAL’s Ca++-free High Salt Barth: Egg Laying (8X)
___________________(1x solution)___________________________amounts to make 8X stock_________________
_______________________________________________2 litres___________4 litres____________8 liters_________
NaCl_________________110 mM___________________102.85g___________205.71g___________308.55g________
Tris base______________15 mM____________________29.075g___________58.15g____________87.23g________
KCl____________________2 mM____________________2.385g_____________4.77g____________7.2g________
Na2HPO4 (anhydrous)___0.5 mM____________________1.36g______________2.72g____________4.1g________
NaHCO3________________2 mM____________________2.69g______________5.38g____________8.07g________
- Adjust pH to 7.6 with glacial acetic acid (approx. 25 mls for 4 litres)
MgSO4.7H2O____________1mM____________________7.89g______________3.945g___________11.84g________
Make 8X stock, dilute to 1x solution in dH2O just before use. Maximum recommended storage: 8 weeks at 4oC.