LEL - all pronephric epithelia- also faint lateral line: Difference between revisions

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(Created page with ''''Lycopersicon Esculentum (Tomato) Lectin (LEL)''' Both Fluorescein (FL-1171 ) and Texas Red conjugates are available from Vector Laboratories [http://www.vectorlabs.com/cata…')
 
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Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples.
Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples.
[[File:Example.jpg]]
[[File:FLEL.jpg]]

Revision as of 16:23, 12 January 2010

Lycopersicon Esculentum (Tomato) Lectin (LEL)


Both Fluorescein (FL-1171 ) and Texas Red conjugates are available from Vector Laboratories [1]

Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples.