LEL - all pronephric epithelia- also faint lateral line: Difference between revisions
Jump to navigation
Jump to search
imported>Pvize (Created page with ''''Lycopersicon Esculentum (Tomato) Lectin (LEL)''' Both Fluorescein (FL-1171 ) and Texas Red conjugates are available from Vector Laboratories [http://www.vectorlabs.com/cata…') |
imported>Pvize No edit summary |
||
Line 6: | Line 6: | ||
Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples. | Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples. | ||
[[File: | [[File:FLEL.jpg]] |
Revision as of 16:23, 12 January 2010
Lycopersicon Esculentum (Tomato) Lectin (LEL)
Both Fluorescein (FL-1171 ) and Texas Red conjugates are available from Vector Laboratories [1]
Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples.