LEL - all pronephric epithelia- also faint lateral line: Difference between revisions
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Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples. | Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples. | ||
[[ | [[Image:FLEL.jpg|thumb|alt=ALT TEXT|Stage 35/36 Xenopus laevis embryos stained with Texas Red conjugated LEL (Image from P. Vize [http://www.xenbase.org/community/person.do?method=display&personId=702&tabId=0])]] | ||
Stage 35/36 Xenopus laevis embryos stained with Texas Red conjugated LEL (Image from P. Vize [http://www.xenbase.org/community/person.do?method=display&personId=702&tabId=0]) | |||
Revision as of 16:36, 12 January 2010
Lycopersicon Esculentum (Tomato) Lectin (LEL)
Both Fluorescein (FL-1171 ) and Texas Red (TL-1176) conjugates are available from Vector Laboratories [2]
Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples.
