LEL - all pronephric epithelia- also faint lateral line

From XenWiki
Jump to navigation Jump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Lycopersicon Esculentum (Tomato) Lectin (LEL)


Both Fluorescein (FL-1171 ) and Texas Red (TL-1176) conjugates are available from Vector Laboratories [2]

Incubate overnight at 4 degrees with 1/100 labeled lectin in standard antibody blocking solution. Wash 4 x 20 min in TBS and photograph. Can be combined with antibody staining incubations. Works on embryos post in situ so can be used as a counter-stain on already processed samples.


option (Image from P. Vize [3])]]

option (Image from P. Vize [4])]]


ALT TEXT
Stage 38/39 Xenopus laevis embryos stained with fluorescein conjugated LEL (Image from P. Vize [1])