Polyclonal anti-phospho-H3 - mitotic cells: Difference between revisions
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'''Anti-phospho-Histone H3 (Ser10)''' | |||
Generated and characterized by XXX [http://www.xenbase.org:80/community/person.do?method=display&personId=XXX] | Generated and characterized by XXX [http://www.xenbase.org:80/community/person.do?method=display&personId=XXX] | ||
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'''Subtype:''' IgG | '''Subtype:''' IgG | ||
'''Antigen:''' histone H3 | '''Antigen:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3 | ||
'''Fixatives:''' | '''Fixatives:''' MEMFA | ||
'''Sensitive to bleaching:''' No | '''Sensitive to bleaching:''' No | ||
| Line 16: | Line 18: | ||
'''Functions post in situ?:''' Yes | '''Functions post in situ?:''' Yes | ||
'''Immunoprecipitation/Western blotting:''' | '''Immunoprecipitation/Western blotting:''' Yes, very good, 17kD target | ||
'''Available as:''' | '''Available as:''' 1 mg/ml solution. Working dilution approx 1/500 | ||
'''Available from:''' | '''Available from:''' Upstate, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570] | ||
'''Optimized conditions:''' | '''Optimized conditions:''' | ||
'''Other species:''' | '''Other species:''' Pretty much everything from tetrahymena to humans | ||
Revision as of 16:56, 14 January 2010
Anti-phospho-Histone H3 (Ser10)
Generated and characterized by XXX [1]
Antibody name: XXX
Raised in: rabbit
Subtype: IgG
Antigen: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
Fixatives: MEMFA
Sensitive to bleaching: No
Functions post in situ?: Yes
Immunoprecipitation/Western blotting: Yes, very good, 17kD target
Available as: 1 mg/ml solution. Working dilution approx 1/500
Available from: Upstate, Cat # 06-570 [2]
Optimized conditions:
Other species: Pretty much everything from tetrahymena to humans