Polyclonal anti-phospho-H3 - mitotic cells: Difference between revisions

Revision as of 16:58, 14 January 2010

Generated and characterized by Hendzel et al. [1]

Antibody name: Anti-phospho-Histone H3 (Ser10)

Raised in: rabbit

Subtype: IgG

Antigen: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Fixatives: MEMFA

Sensitive to bleaching: No

Functions post in situ?: Yes

Immunoprecipitation/Western blotting: Yes, very good, 17kD target

Available as: 1 mg/ml solution. Working dilution approx 1/500

Available from: Upstate, Cat # 06-570 [2]

Optimized conditions:

Other species: Pretty much everything from tetrahymena to humans

@@ Line 1: / Line 1: @@
-'''Anti-phospho-Histone H3 (Ser10)'''
-Generated and characterized by XXX [http://www.xenbase.org:80/community/person.do?method=display&personId=XXX]
+Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543]
-'''Antibody name:''' XXX
+'''Antibody name:''' Anti-phospho-Histone H3 (Ser10)
 '''Raised in:''' rabbit