Polyclonal anti-phospho-H3 - mitotic cells

Generated and characterized by Hendzel et al. [1] First use in Xenopus- Saka and Smith 2001 [2]

Antibody name: Anti-phospho-Histone H3 (Ser10)

Raised in: rabbit

Subtype: IgG

Antigen: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Fixatives: MEMFA

Sensitive to bleaching: No

Functions post in situ?: Yes

Immunoprecipitation/Western blotting: Yes, very good, 17kD target

Available as: 1 mg/ml solution.

Available from: Upstate, Cat # 06-570 [3]

Optimized conditions: Working dilution approx 1/500

Other species: Pretty much everything from tetrahymena to humans

Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)