Protocols

• Animal Protocols

• • Inducing egg laying via hCG injection

• Transgenesis

• Affinity Columns
  • Making an Affinity Column
  • Running an Affinity Column

• Oocyte Transfer Technique
  • Part 1: Choosing Oligodeoxyribonucleotides (ODNs)
  • Part 2: ODN Modification
  • Part 3: Host Transfer Technique
  • Part 4:Fertilization and Development

• Fluorescent In-Situs and FCIS
  • Wholemount embryo FISH / Double FISH
  • Flourescin Tyramide Synthesis
  • Cy3 Tyramide Synthesis
  • FCIS
  • Chromosomal FISH-TSA

• Embryo staining protocols
  • Apoptosis via TUNEL
  • Benzidine-peroxidase staining for blood
  • In situ hybridization
  • Wholemount antibody staining