Smad 1/5/8, phosphorylated - smad1 - smad8: Difference between revisions

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(Created page with "'''Antigen:''' human phospho-smad1/smad5/smad8 '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=1018192 smad1] [http://www.xenbase.org...")
 
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'''Antigen:''' human phospho-smad1/smad5/smad8
'''Antigen:''' human phospho-smad1/smad5/smad8
 
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=1018192 smad1] [http://www.xenbase.org/gene/showgene.do?method=display&geneId=484685 smad8]
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=1018192 smad1] [http://www.xenbase.org/gene/showgene.do?method=display&geneId=484685 smad8]
'''Epitope:'''
'''Epitope:'''
 
'''Host Species/Isotype:''' rabbit polyclonal
'''Host Species/Isotype:''' rabbit polyclonal
 
'''Antigen Species:''' human
'''Antigen Species:''' human
'''Applications:''' WB
'''Applications:''' IF, WB
 
'''Antibody Name/Catalog Number:''' phospho-smad1/smad5/smad8 antibody [http://www.cellsignal.com/products/9511.html #9511]
'''Antibody Name/Catalog Number:''' phospho-smad1/smad5/smad8 antibody [http://www.cellsignal.com/products/9511.html #9511]
 
'''Supplier:''' cell signalling
'''Supplier:''' cell signalling
 
'''Comments:''' works reasonably well on WB
'''Comments:''' IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, use at 1:500 WB: use at 1:1000-1:5000
 
'''Contributor:''' Rachel Stephenson
'''Contributor:''' Zorn Lab (IF) Rachel Stephenson (WB)
 
'''Additional Information:'''
'''Additional Information:'''
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IP - immunoprecipitation,
IP - immunoprecipitation,
 
IF - immunofluorescence,
IF - immunofluorescence,
 
IHC - immunohistochemistry,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry
FCM - flow cytometry

Latest revision as of 09:12, 9 March 2011

Antigen: human phospho-smad1/smad5/smad8

Xenbase Gene Page: smad1 smad8

Epitope:

Host Species/Isotype: rabbit polyclonal

Antigen Species: human

Applications: IF, WB

Antibody Name/Catalog Number: phospho-smad1/smad5/smad8 antibody #9511

Supplier: cell signalling

Comments: IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, use at 1:500 WB: use at 1:1000-1:5000

Contributor: Zorn Lab (IF) Rachel Stephenson (WB)

Additional Information:

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry