Collagen type II - col2a1 - ECM broad and Phospho-histone H3 - h3f3a - mitotic cells - from upstate: Difference between pages

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(Created page with "'''Antigen:''' phosporylated histone H3 (Ser10) '''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3 '''Host Sp...")
 
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'''Antigen:''' chicken type II collagen
'''Antigen:''' phosporylated histone H3 (Ser10)
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=6258352 col2a1]
'''Epitope:''' KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3
'''Epitope:'''
'''Host Species/Isotype:'''
'''Host Species/Isotype:''' mouse monoclonal IgG2a
'''Antigen Species:''' human
'''Antigen Species:''' chicken
'''Applications:''' IF, WB
'''Applications:''' IF
'''Antibody Name:'''
'''Antibody Name/Catalog Number:''' [http://dshb.biology.uiowa.edu/collagen-type-II CIIC1]
'''Supplier:''' Upstate/Millipore, Cat # 06-570 [http://www.millipore.com/catalogue/item/06-570]
'''Supplier:''' Developmental Studies Hybridoma Bank
'''Comments:''' not sentitive to bleaching, works post in situ
'''Comments:''' works for IF but not well, fixative: Dent's
'''Contributor:''' Generated and characterized by Hendzel et al. [http://www.ncbi.nlm.nih.gov/pubmed/9362543] First use in Xenopus- Saka and Smith 2001 [http://www.xenbase.org/literature/article.do?method=display&articleId=9731]
'''Contributor:''' Sandra Kolker and Dan Weeks
'''Additional Information:'''
'''Additional Information:'''
'''Reference(s):'''
'''Reference(s):'''

Latest revision as of 08:18, 8 March 2011

Antigen: phosporylated histone H3 (Ser10)

Epitope: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Host Species/Isotype:

Antigen Species: human

Applications: IF, WB

Antibody Name:

Supplier: Upstate/Millipore, Cat # 06-570 [1]

Comments: not sentitive to bleaching, works post in situ

Contributor: Generated and characterized by Hendzel et al. [2] First use in Xenopus- Saka and Smith 2001 [3]

Additional Information:

Reference(s):



applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry