Active b-catenin - ctnnb1 and Alpha-catenin - ctnna1: Difference between pages

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(Created page with "'''Antigen:''' human b-catenin '''Epitope:''' aa 36-44 '''Host Species/Isotype:''' mouse monoclonal IgG1 clone 8E7 '''Antigen Species:''' human '''Applications:''...")
 
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'''Antigen:''' human b-catenin
'''Antigen:''' human/mouse alpha-catenin
'''Epitope:''' aa 36-44
'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=479598 ctnna1]
'''Host Species/Isotype:''' mouse monoclonal IgG1 clone 8E7
'''Epitope:''' aa 890-901
'''Host Species/Isotype:''' rabbit
'''Antigen Species:''' human
'''Antigen Species:''' human
'''Applications:''' WB
'''Applications:''' IF, WB
'''Antibody Name:''' anti-ABC 05-665 [http://www.millipore.com/catalogue/item/05-665]
'''Antibody Name/Catalog Number:''' [http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=C2081|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC anti-a-catenin C 2081]
'''Supplier:''' Upstate Cell Signaling Solutions/Millipore
'''Supplier:''' Sigma
'''Comments:''' WB: use at 1:1000
'''Comments:''' IF: MEMFA 1-2 hr @ RT, post-fix in Dent's, use at 1:300 WB: use at 1:5000
'''Contributor:''' Zorn Lab
'''Contributor:''' Zorn Lab
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'''Reference(s):'''
'''Reference(s):'''

Latest revision as of 15:12, 8 March 2011

Antigen: human/mouse alpha-catenin

Xenbase Gene Page: ctnna1

Epitope: aa 890-901

Host Species/Isotype: rabbit

Antigen Species: human

Applications: IF, WB

Antibody Name/Catalog Number: anti-a-catenin C 2081

Supplier: Sigma

Comments: IF: MEMFA 1-2 hr @ RT, post-fix in Dent's, use at 1:300 WB: use at 1:5000

Contributor: Zorn Lab

Additional Information:

Reference(s):


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry