C-myc - myc - from roche and Active JNK - mapk8: Difference between pages

Latest revision as of 09:06, 8 March 2011

Antigen: active Janus kinase

Epitope: dually phosphorylated JNK-derived peptide

Host Species/Isotype: rabbit polyclonal

Antigen Species:

Applications: IF

Antibody Name: anti-active JNK pAb V7931 [1]

Supplier: Promega

Comments: IF: MEMFA 30 min @ RT, post-fix Dent's (80% MeOH/20% DMSO), use @ 1:300

Contributor: Zorn Lab

Additional Information:

Reference(s):

applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

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-'''Antigen:'''		E-cadherin
+'''Antigen:'''		active Janus kinase
-'''Xenbase Gene Page:'''		[http://www.xenbase.org/gene/showgene.do?method=display&geneId=12531593 cdh1]
+'''Epitope:'''		dually phosphorylated JNK-derived peptide
-'''Epitope:'''		140 kDa xenopus E-cadherin
+'''Host Species/Isotype:'''		rabbit polyclonal
-'''Host Species/Isotype:'''		mouse monoclonal IgG2b
+'''Antigen Species:'''
-'''Antigen Species:'''		xenopus
 '''Applications:'''		IF
-'''Antibody Name/Catalog Number:'''		[http://dshb.biology.uiowa.edu/cadherin-E- 5D3]
+'''Antibody Name:'''		anti-active JNK pAb V7931 [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1166]
-'''Supplier:'''		Developmental Systems Hybridoma Bank
+'''Supplier:'''		Promega
-'''Comments:'''		IF: fix directly in Dent's 1-2 hrs then into 100% methanol use at 1:300
+'''Comments:'''		IF: MEMFA 30 min @ RT, post-fix Dent's (80% MeOH/20% DMSO), use @ 1:300
-'''Contributor:'''		Zorn Lab and V Ponferrada
+'''Contributor:'''		Zorn Lab
-'''Additional Information:'''		MEMFA, 10% TCA and 4% PFA in 1x PBS fixatives also work
+'''Additional Information:'''
 '''Reference(s):'''