P44/42 MAPK, ERK1/2 phosphorylated - mapk1 - from cell signaling technology and Recipe: Difference between pages

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(Created page with "'''Antigen:''' phospho-p44/42 MAPK '''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=479466 mapk1] '''Epitope:''' phosphorylated p44/...")
 
(Created page with "10 mm HEPES 60 mm NaCl 0.67 mm KCl 0.34 mm Ca(NO3)2 0.83 mm MgSO4 Adjust to pH 7.4. * © 2011 Cold Spring Harbor Laboratory Press")
 
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'''Antigen:''' phospho-p44/42 MAPK
10 mm HEPES


'''Xenbase Gene Page:''' [http://www.xenbase.org/gene/showgene.do?method=display&geneId=479466 mapk1]
60 mm NaCl
'''Epitope:''' phosphorylated p44/42 MAPK at individually or dually at Thr202/Tyr204


'''Host Species/Isotype:''' rabbit
0.67 mm KCl


'''Antigen Species:''' sythetic peptide of residues surrounding Thr202/Tyr204 of human MAPK
0.34 mm Ca(NO3)2
'''Applications:''' IF


'''Antibody Name/Catalog Number:''' [http://www.cellsignal.com/products/9101.html phospho-p44/42 MAPK antibody #9101]
0.83 mm MgSO4


'''Supplier:''' Cell Signaling Techonology
Adjust to pH 7.4.


'''Comments:''' IF: MEMFA 1-2 hrs @ RT, post-fix Dent's, use at 1:400
    * © 2011 Cold Spring Harbor Laboratory Press
 
'''Contributor:''' Zorn Lab
 
'''Additional Information:'''
'''Reference(s):'''
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
 
IF - immunofluorescence,
 
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
 
ChIP - chromatin immunoprecipitation,
 
FCM - flow cytometry

Latest revision as of 13:09, 20 October 2011

10 mm HEPES

60 mm NaCl

0.67 mm KCl

0.34 mm Ca(NO3)2

0.83 mm MgSO4

Adjust to pH 7.4. 

   * © 2011 Cold Spring Harbor Laboratory Press
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