Wholemount antibody staining (CSH Protocols) (Wallingford lab)

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Revision as of 09:47, 15 November 2011 by imported>Virgilio (moved Wholemount antibody staining to Wallingford Lab - wholemount antibody staining (CSH Protocols): added lab attribution)
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There are currently several published protocols for this. We (Wallingford lab) have recently published a protocol in CSH Protocols:

http://cshprotocols.cshlp.org/cgi/content/full/2008/3/pdb.prot4957?maxtoshow=&hits=10&RESULTFORMAT=&fulltext=wallingford&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT

That protocol draws from the many excellent previous protcols including:


Robinson, C. and Guille, M. 1999. Immunohistochemistry of Xenopus embryos. Methods Mol. Biol. 127: 89–97.

Sive, H.L., Grainger, R.M., and Harland, R.M. 2000. Early development of Xenopus laevis: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Becker, B.E and Gard, D.L. 2006. Visualization of the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy. Methods Mol. Biol. 322: 69–86.

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