Lab Solutions and Reagents

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Revision as of 13:51, 6 December 2011 by imported>Virgilio
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  • recipes for miscellaneous salts, solutions, etc. (Khokha lab)
    • agarose plates 1% - for use with laevis or tropicalis
    • Alkaline Phosphatase (AP) Buffer, with Levamisol and Tween
    • amphibian Ringers, 5 gal (19 L)
    • bleaching solution (Sive et al. 2000, Mayor et al. 1995)
    • 4% BMB blocking reagent in MAB
    • Bouin’s fixative
    • cysteine, 2.0%
    • dehydrating embryos
    • Denhardt’s solution, 100X
    • dNTPs 10 mM
    • 2.5% Ficoll
    • 2.5% Ficoll in 1/3X MR (for use with X. laevis embryos)
    • 3% Ficoll in 1/9XMR (for use with X.tropicalis embryos)
    • gentamicin stock, Sigma (1000X, 100 mg/ml)
    • HEPES 1 M, pH 7.4
    • hybridization buffer
    • levamisol 1 M
    • MAB, 10X
    • 1M MgCl2
    • MEMFA* (Sive et al. 2000)
    • 100X MR salts
    • 1/3X Modified Ringers
    • 1/9X MR + 1% Gentamicin
    • 1/20X MR
    • modified Ringers
    • paraformaldehyde(PFA), 20%
    • phosphate buffered saline (PBS), 10X
    • proteinase K(PK), 10 mg/ml
    • PTw
    • rehydrating embryos (for embryos stored in 100% methanol)
    • RNase A (Sigma R 5000)
    • RNase T1 (Sigma R8251)
    • 4M NaCl, 1 liter
    • SDS 10%
    • Sodium Phosphate Buffer(1M)
    • SSC, 20X
    • TAE 50X
    • Triethanolamine 0.1 M (pH7.0-8.0)
    • 1M Tris pH 9.5