Monoclonal 12/101 - somites: Difference between revisions
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Created page with 'Generated and characterized by Kintner [http://www.xenbase.org:80/community/person.do?method=display&personId=1135] and Brockes (1985) [http://www.xenbase.org/literature/article.…' |
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'''Sensitive to bleaching:''' No | '''Sensitive to bleaching:''' No | ||
'''Functions post in situ?:''' Yes | |||
'''Immunoprecipitation/Western blotting:''' 120,000 Mr target | '''Immunoprecipitation/Western blotting:''' 120,000 Mr target | ||
| Line 18: | Line 20: | ||
'''Available as:''' cell supernatant, 3 x or as ascites | '''Available as:''' cell supernatant, 3 x or as ascites | ||
'''Available from:''' Developmental Studies Hybridoma Bank | '''Available from:''' Developmental Studies Hybridoma Bank [http://dshb.biology.uiowa.edu/skeletal-muscle-marker] | ||
'''Optimized conditions:''' Very flexible | |||
'''Other species:''' Many- chick, mouse, newt, rabbit | |||
[[File:12101.jpg]] | |||
Immunofluorescence using 12/101 | |||
[[File:12101+ATPase.jpg]] | |||
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green. | |||
Latest revision as of 15:29, 14 January 2010
Generated and characterized by Kintner [1] and Brockes (1985) [2]
Antibody name: 12/101
Raised in: mouse
Subtype: IgG
Antigen: homogenized tissue
Fixatives: MEMFA, Dent's, TCA
Sensitive to bleaching: No
Functions post in situ?: Yes
Immunoprecipitation/Western blotting: 120,000 Mr target
Available as: cell supernatant, 3 x or as ascites
Available from: Developmental Studies Hybridoma Bank [3]
Optimized conditions: Very flexible
Other species: Many- chick, mouse, newt, rabbit
Immunofluorescence using 12/101
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.