4A6 – pronephros: Difference between revisions

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Created page with "'''Antigen:''' dissected pronephroi, target unknown '''Epitope:''' '''Host Species/Isotype:''' mouse monoclonal IgG '''Antigen Species:''' '''Applicatio..."
 
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'''Antigen:''' dissected pronephroi, target unknown
'''Antigen:''' dissected pronephroi, target unknown
'''Epitope:'''
'''Xenbase Gene Page:'''
'''Host Species/Isotype:''' mouse monoclonal IgG
'''Epitope:''' mouse monoclonal IgG
'''Antigen Species:'''
'''Host Species/Isotype:'''
'''Applications:''' IF, IP, WB
'''Antigen Species:'''
'''Antibody Name:''' 4A6
'''Applications:''' IF, IP, WB
'''Supplier:''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.
'''Antibody Name/Catalog Number:''' 4A6
'''Comments:''' not sentitive to bleaching, does not function post in situ
'''Supplier:''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.
'''Contributor:''' Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]
'''Comments:''' not sentitive to bleaching, does not function post in situ
 
'''Contributor:''' Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]
applications abbreviations:
WB - Western blotting,
[[File:4A6.jpg]]
IP - immunoprecipitation,
IF - immunofluorescence,
'''Additional Information:'''
IHC - immunohistochemistry,
'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.
EMSA - electromobility shift assay,
''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.
ChIP - chromatin immunoprecipitation,
'''Fixatives:''' MEMFA
FCM - flow cytometry
''Available as:''' cell supernatant, 3 x
 
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense.  As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.
Additional Comments:
'''Other species:''' Xenopus only
'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested.
'''Reference(s):''' Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]
''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias.
'''Fixatives:''' MEMFA
''Available as:''' cell supernatant, 3 x
applications abbreviations:
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense.  As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos.
'''Other species:''' Xenopus only
WB - Western blotting,
 
References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219]
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry

Latest revision as of 15:03, 8 March 2011

Antigen: dissected pronephroi, target unknown

Xenbase Gene Page:

Epitope: mouse monoclonal IgG

Host Species/Isotype:

Antigen Species:

Applications: IF, IP, WB

Antibody Name/Catalog Number: 4A6

Supplier: Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.

Comments: not sentitive to bleaching, does not function post in situ

Contributor: Generated and characterized by Elizabeth Jones [1]. References: Dev Bio 171:531- [2]

Additional Information: Developmental Window: First detects target at stage 39, continues until latest stages tested. Stains:' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias. Fixatives: MEMFA Available as:' cell supernatant, 3 x Optimized conditions: Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. Other species: Xenopus only

Reference(s): Dev Bio 171:531- [3]


applications abbreviations:

WB - Western blotting,

IP - immunoprecipitation,

IF - immunofluorescence,

IHC - immunohistochemistry,

EMSA - electromobility shift assay,

ChIP - chromatin immunoprecipitation,

FCM - flow cytometry

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