Protocols

• Animal Protocols
  • Inducing egg laying via hCG injection

• Purifying Polyclonal Antibodies using Affinity Columns / Chromatography
  • Making an Affinity Column
  • Running an Affinity Column

• Oocyte Transfer Technique
  • Part 1: Choosing Oligodeoxyribonucleotides (ODNs)
  • Part 2: ODN Modification
  • Part 3: Host Transfer Technique
  • Part 4:Fertilization and Development

• Fluorescent In-Situs and FCIS
  • Wholemount embryo FISH / Double FISH
  • Flourescin Tyramide Synthesis
  • Cy3 Tyramide Synthesis
  • FCIS
  • Chromosomal FISH-TSA

• Embryo Staining Protocols
  • Apoptosis via TUNEL
  • Benzidine-peroxidase staining for blood
  • In situ hybridization
  • Wholemount antibody staining

• Xenopus Oocyte and Egg Extracts
  • Crude interphase egg extract ("LSS" - low speed supernatant)
  • Isolation of chromatin from egg extract and histone recovery
  • Preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase)
  • Cycling Extract (2)
  • Cycling Extract (3)
  • CSF Extract (1)
  • CSF Extract (2)
  • Assaying H1 kinase in extracts

• Xenopus Tissue Culture
  • Tissue culture of Xenopus cell lines S3, XTC
  • Isolation of mitotic chromosomes from XTC cells
  • In-vitro kinetochore-microtubule binding assays
  • Immunofluorescence with biotinylated antibodies

• Other Protocols
  • Immunoprecipitation
  • Stukenberg Lab - silver staining protein gels