Chromosomal FISH-TSA

Literature:

Krylov V., Macha J., Tlapakova T., Takac M., Jonak J. (2003) The c-SRC1 gene visualized by in situ hybridization on Xenopus laevis chromosomes. Cytogenet. Genome Res. 2003;103(1-2):169-172. [1].

Tlapakova T., Krylov V., Macha J. (2005) Localization, structure and polymorphism of two paralogous Xenopus laevis mitochondrial malate dehydrogenase genes. Chromosome res. 13(7):699-706. [2].

Krylov V., Tlapakova T., Macha J. (2007) Localization of Mdh2 single copy gene on Xenopus tropicalis chromosomes by FISH-TSA technique. Cytogenet. Genome Res. 116:110-112. [3].

Following protocol is designed for the visualization of short cDNA sequences with the minimal lenght of 900-1000 bp.

Protocol:

• File:Krylov FISH-TSA protocol.pdf