Protocols: Difference between revisions
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*'''Animal Protocols''' | *'''Animal Husbandry/Animal Protocols''' | ||
**[[Inducing egg laying via hCG injection]] | **[[Inducing egg laying via hCG injection]] | ||
*''' | *'''Embryo Staining Protocols''' | ||
**[[ | **[[Harland Lab - in situ hybridization (brief version)]] | ||
**[[Thomsen Lab - benzidine-peroxidase staining for blood]] | |||
**[[Veenstra Lab - apoptosis via TUNEL]] | |||
**[[Wallingford Lab - wholemount antibody staining (CSH Protocols)]] | |||
**[[ | |||
**[[ | |||
**[[ | |||
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*''' | *'''[[Heasman Lab - Oocyte Transfer Technique]]''' | ||
**[[ | **[[Part 1: Choosing Oligodeoxyribonucleotides (ODNs)]] | ||
**[[ | **[[Part 2: ODN Modification]] | ||
**[[ | **[[Part 3: Host Transfer Technique]] | ||
**[[ | **[[Part 4:Fertilization and Development]] | ||
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**[[Stukenberg Lab - in-vitro kinetochore-microtubule binding assays]] | **[[Stukenberg Lab - in-vitro kinetochore-microtubule binding assays]] | ||
**[[Stukenberg Lab - immunofluorescence with biotinylated antibodies]] | **[[Stukenberg Lab - immunofluorescence with biotinylated antibodies]] | ||
*'''Purifying Polyclonal Antibodies using Affinity Columns / Chromatography''' | |||
**[[Making an Affinity Column]] | |||
**[[Running an Affinity Column]] | |||
Revision as of 11:18, 15 November 2011
- Animal Husbandry/Animal Protocols
- Embryo Staining Protocols
- Xenopus Oocyte and Egg Extracts
- Shechter Lab - crude interphase egg extract ("LSS" - low speed supernatant)
- Shechter Lab - isolation of chromatin from egg extract and histone recovery
- Shechter Lab - preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase)
- Stukenberg Lab - Mike's cycling extracts
- Stukenberg Lab - Sara's cycling extracts
- Stukenberg Lab - Mike's CSF extract
- Stukenberg Lab - Sara's CSF extract
- Stukenberg Lab - assaying H1 kinase in extracts
- Xenopus Tissue Culture
- Purifying Polyclonal Antibodies using Affinity Columns / Chromatography