Antibodies and lectins

Antibodies

Antibody template

12/101 – somites (monoclonal)

3G8- pronephros and otic vesicle (monoclonal)

4A6 – pronephros (monoclonal)

Centrin 1 - basal bodies

phospho-histone H3 - mitotic cells

troponin - heart and other muscle

troponin - cardiac troponin T - tnnt2 - heart

alpha-tubulin - microtubules

gamma-tubulin - Basal bodies, microtubule minus ends

ZO-1

Lectins

ECL - pronephric nephrons

LEL - all pronephric epithelia- also faint lateral line

PNA - cement gland and scattered epidermal cells