Protocols: Difference between revisions
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**[[in vitro fertilization of Xenopus laevis (Conlon lab)]] | **[[in vitro fertilization of Xenopus laevis (Conlon lab)]] | ||
**[[high-salt solution for egg laying (Zorn lab/Ron Laskey)]] | **[[high-salt solution for egg laying (Zorn lab/Ron Laskey)]] | ||
**[[dejellying embryos (Sive et al. 2000)]] | |||
**[[dejellying embryos (Zorn lab)]] | **[[dejellying embryos (Zorn lab)]] | ||
Revision as of 11:45, 16 December 2011
- Books for Xenopus Research and Protocols
- Normal Table of Xenopus Laevis (Daudin), Nieuwkoop and Faber (Third edition), 1994 [1]
- a systematic and chronological description of Xenopus laevis development
- Early Development of Xenopus laevis: A Laboratory Manual, Sive, Grainger, and Harland, 2000 [2]
- comprehensive collection of protocols for the study of early development in Xenopus embryos
- Xenopus Protocols: Cell Biology and Signal Transduction, Liu (First edition), 2006 [3]
- step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
- Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36, Kay and Peng, 1991 [4]
- detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
- Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology, Seidman and Soreq, 1997 [5]
- referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
- Atlas of Xenopus Development, Bernardini, Prati, Bonetti, and Scari, 1999 [6]
- scanning, transmission, and light microscopy images of Xenopus embryonic development
- The early development of Xenopus laevis. An atlas of the histology, Hausen and Riebesell, 1991 [7]
- detailed histological sections of Xenopus embryonic development
- Color Atlas of Xenopus laevis Histology, Wiechmann and Wirsig-Wiechmann, 2003 [8]
- central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis
- Normal Table of Xenopus Laevis (Daudin), Nieuwkoop and Faber (Third edition), 1994 [1]
- Lab Solutions and Reagents - click to view expanded list
- Animal Husbandry/Animal Protocols
- inducing egg laying via hCG injection (Vize lab)
- anesthesia, in vitro fertilization, and natural mating of Xenopus tropicalis (Conlon lab)
- benzocaine stock, 100x (Khokha lab)
- in vitro fertilization of Xenopus laevis (Conlon lab)
- high-salt solution for egg laying (Zorn lab/Ron Laskey)
- dejellying embryos (Sive et al. 2000)
- dejellying embryos (Zorn lab)
- Transgenics
- In Situ Hybridization
- Immunohisochemistry
- wholemount antibody staining (CSH Protocols) (Wallingford lab)
- wholemount antibody staining - alkaline phosphatase detection (Conlon lab)
- wholemount antibody staining - adapted from Dan Weeks' protocol (Conlon lab)
- immunostaining sectioned embryos (Conlon lab)
- hydrochloric acid antigen retrieval (Zorn lab)
- Other Embryo Staining Protocols
- Other Immuno Protocols and Protein Protocols
- immunoprecipitation (Stukenberg lab)
- immunofluorescence with biotinylated antibodies (Stukenberg lab)
- Purifying Polyclonal Antibodies using Affinity Columns/Chromatography
- whole embryo protein extraction with freon (Conlon lab)
- SDS-PAGE gels (Conlon lab)
- western blot protocol (Conlon lab)
- silver staining protein gels (Stukenberg lab)
- Xenopus Oocyte and Egg Extracts
- crude interphase egg extract (Shechter lab)
- isolation of chromatin from egg extract and histone recovery (Shechter lab)
- preparation of cycling egg extract (Shechter lab)
- cycling extracts, mike's protocol (Stukenberg lab)
- cycling extracts, sara's protocol (Stukenberg lab)
- CSF extract, mike's protocol (Stukenberg lab)
- CSF extract, sara's protocol (Stukenberg lab)
- assaying H1 kinase in extracts (Stukenberg lab)