Wholemount antibody staining (CSH Protocols) (Wallingford lab)

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There are currently several published protocols for this. We (Wallingford lab) have recently published a protocol in CSH Protocols:


That protocol draws from the many excellent previous protcols including:

Robinson, C. and Guille, M. 1999. Immunohistochemistry of Xenopus embryos. Methods Mol. Biol. 127: 89–97.

Sive, H.L., Grainger, R.M., and Harland, R.M. 2000. Early development of Xenopus laevis: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Becker, B.E and Gard, D.L. 2006. Visualization of the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy. Methods Mol. Biol. 322: 69–86.