4A6 – pronephros: Difference between revisions
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'''Antigen:''' | '''Antigen:''' dissected pronephroi, target unknown | ||
'''Epitope:''' | '''Epitope:''' | ||
'''Host Species/Isotype:''' | '''Host Species/Isotype:''' mouse monoclonal IgG | ||
'''Antigen Species:''' | '''Antigen Species:''' | ||
'''Applications:''' | '''Applications:''' IF, IP, WB | ||
'''Antibody Name:''' | '''Antibody Name:''' 4A6 | ||
'''Supplier:''' | '''Supplier:''' Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs. | ||
'''Comments:''' | '''Comments:''' not sentitive to bleaching, does not function post in situ | ||
'''Contributor:''' | '''Contributor:''' Generated and characterized by Elizabeth Jones [http://www.xenbase.org:80/community/person.do?method=display&personId=753]. References: Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219] | ||
'''Additional Information:''' | |||
'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested. | |||
''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias. | |||
'''Fixatives:''' MEMFA | |||
''Available as:''' cell supernatant, 3 x | |||
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. | |||
'''Other species:''' Xenopus only | |||
'''Reference(s):''' Dev Bio 171:531- [http://www.xenbase.org/literature/article.do?method=display&articleId=19219] | |||
Additional | |||
applications abbreviations: | |||
'''Developmental Window:''' First detects target at stage 39, continues until latest stages tested. | |||
''Stains:''' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias. | WB - Western blotting, | ||
'''Fixatives:''' MEMFA | |||
''Available as:''' cell supernatant, 3 x | IP - immunoprecipitation, | ||
'''Optimized conditions:''' Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. | |||
'''Other species:''' Xenopus only | IF - immunofluorescence, | ||
IHC - immunohistochemistry, | |||
EMSA - electromobility shift assay, | |||
ChIP - chromatin immunoprecipitation, | |||
FCM - flow cytometry | |||
Revision as of 06:55, 8 March 2011
Antigen: dissected pronephroi, target unknown
Epitope:
Host Species/Isotype: mouse monoclonal IgG
Antigen Species:
Applications: IF, IP, WB
Antibody Name: 4A6
Supplier: Liz Jones (ea@dna.bio.warwick.ac.uk) , 10 ml 3x. Sent on dry ice so please provide account number for shipping costs.
Comments: not sentitive to bleaching, does not function post in situ
Contributor: Generated and characterized by Elizabeth Jones [1]. References: Dev Bio 171:531- [2]
Additional Information: Developmental Window: First detects target at stage 39, continues until latest stages tested. Stains:' distal pronephric nephron and pronephric / Wolffian duct. The duct staining is mosaic and only some cells are recognized. Sains the entire cell surface evenly with no basolateral/apical bias. Fixatives: MEMFA Available as:' cell supernatant, 3 x Optimized conditions: Very clean antibody, very stable, can be reused. Development in BCIP/NBT usually takes 30 minutes, in red, about 60 minutes. Works well in immunofluorescense. As the pronephros is only covered by a thin transparent epidermis it is not necessary to clear stained embryos. Other species: Xenopus only
Reference(s): Dev Bio 171:531- [3]
applications abbreviations:
WB - Western blotting,
IP - immunoprecipitation,
IF - immunofluorescence,
IHC - immunohistochemistry,
EMSA - electromobility shift assay,
ChIP - chromatin immunoprecipitation,
FCM - flow cytometry