Monoclonal 12/101 - somites: Difference between revisions
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'''Optimized conditions:''' Very flexible | '''Optimized conditions:''' Very flexible | ||
'''Other species:''' Many- chick, mouse, newt, | '''Other species:''' Many- chick, mouse, newt, rabbit | ||
[[File:Example.jpg]] | |||
Immunofluorescence using 12/101 | |||
[[File:Example.jpg]] | |||
Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green. | |||
Revision as of 15:27, 14 January 2010
Generated and characterized by Kintner [1] and Brockes (1985) [2]
Antibody name: 12/101
Raised in: mouse
Subtype: IgG
Antigen: homogenized tissue
Fixatives: MEMFA, Dent's, TCA
Sensitive to bleaching: No
Functions post in situ?: Yes
Immunoprecipitation/Western blotting: 120,000 Mr target
Available as: cell supernatant, 3 x or as ascites
Available from: Developmental Studies Hybridoma Bank [3]
Optimized conditions: Very flexible
Other species: Many- chick, mouse, newt, rabbit
File:Example.jpg Immunofluorescence using 12/101
File:Example.jpg Example of using 12/101 post in situ as a counterstain. The fluorescent in situ was against atp1a1, which is expressed in the kidney and appears green.