Protocols

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  • Books for Xenopus Research and Protocols
    • Normal Table of Xenopus Laevis (Daudin), Nieuwkoop and Faber (Third edition), 1994 [1]
      • a systematic and chronological description of Xenopus laevis development
    • Early Development of Xenopus laevis: A Laboratory Manual, Sive, Grainger, and Harland, 2000 [2]
      • comprehensive collection of protocols for the study of early development in Xenopus embryos
    • The Laboratory Xenopus sp., Green, 2010 [3]
      • a highly detailed manual containing Xenopus husbandry, management, veterinary care, and frog and equipment vendor information
    • Xenopus Protocols: Cell Biology and Signal Transduction, Liu (First edition), 2006 [4]
      • step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
    • Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36, Kay and Peng, 1991 [5]
      • detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
    • Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology, Seidman and Soreq, 1997 [6]
      • referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
    • Atlas of Xenopus Development, Bernardini, Prati, Bonetti, and Scari, 1999 [7]
      • scanning, transmission, and light microscopy images of Xenopus embryonic development
    • The early development of Xenopus laevis. An atlas of the histology, Hausen and Riebesell, 1991 [8]
      • detailed histological sections of Xenopus embryonic development
    • Color Atlas of Xenopus laevis Histology, Wiechmann and Wirsig-Wiechmann, 2003 [9]
      • central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis


  • Online Resources for Xenopus Research and Protocols
    • Cold Spring Harbor Protocols - Xenopus [10], subscription required
    • The Journal of Visualized Experiments (JOVE) - Xenopus embryo [11] - excellent video demonstrations
      • Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation - Enrique Amaya & Kristen Kroll [12]
      • Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis - Andrea S. Viczian & Michael E. Zuber [13]
      • Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development - Paaqua A. Grant, Mona B. Herold & Sally A. Moody [14]
      • Neural Explant Cultures from Xenopus laevis - Laura Anne Lowery, Anna E.R. Faris, Alina Stout & David Van Vactor [15]
      • Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling - Keiji Itoh & Sergei Y. Sokol [16]
      • Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs - Judith A. Sharp & Mike D. Blower [17]
      • Electroporation of Craniofacial Mesenchyme - Jacqueline M. Tabler & Karen J. Liu [18]
      • Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures - Bruce Yazejian, Rita M. Yazejian, Rachel Einarsson & Alan D. Grinnell [19]
      • Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue - Molly J. McDonough, Chelsea E. Allen, Ng-Kwet-Leok A. Ng-Sui-Hing, Brian A. Rabe, Brittany B. Lewis & Margaret S. Saha [20]
      • Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis - Sagar D. Joshi & Lance A. Davidson [21]
      • Plastic Embedding and Sectioning of Xenopus laevis Embryos - Souichi Ogata1, Shimako Kawauchi, Anne Calof & Ken W.Y. Cho [22]
      • Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay - Souichi Ogata & Ken W.Y. Cho [23]
      • Fertilization of Xenopus oocytes using the Host Transfer Method - Patricia N. Schneider, Alissa M. Hulstrand & Douglas W. Houston [24]
      • Microinjection of Xenopus Laevis Oocytes - Sarah Cohen, Shelly Au & Nelly Panté - [25]
      • Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis - Hristina Nedelkovska, Tanya Cruz-Luna, Pamela McPherson & Jacques Robert [26]
      • Two Types of Assays for Detecting Frog Sperm Chemoattraction - Lindsey A. Burnett, Nathan Tholl & Douglas E. Chandler [27]
      • Study of the DNA Damage Checkpoint using Xenopus Egg Extracts - Jeremy Willis, Darla DeStephanis, Yogin Patel, Vrushab Gowda & Shan Yan [28]
      • Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes - Austin L Brown, Brandon E. Johnson & Miriam B. Goodman [29]
      • Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays - Katharina L. Dürr, Neslihan N. Tavraz, Susan Spiller & Thomas Friedrich [30]
      • Preparation and Fractionation of Xenopus laevis Egg Extracts - Marie K. Cross & Maureen Powers [31]
      • Obtaining Eggs from Xenopus laevis Females - Marie K. Cross & Maureen Powers [32]
      • In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts - Marie Cross & Maureen Powers [33]
      • Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes - Junqiu Yang, Kelli Delaloye, Urvi S. Lee & Jianmin Cui [34]
      • Visualizing RNA Localization in Xenopus Oocytes - James A. Gagnon & Kimberly L. Mowry [35]
      • Single Cell Electroporation in vivo within the Intact Developing Brain - D. Sesath Hewapathirane & Kurt Haas - [36]














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