Protocols

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Books for Xenopus Research and Protocols

  • Normal Table of Xenopus Laevis (Daudin), Nieuwkoop and Faber (Third edition), 1994 [1]
    • a systematic and chronological description of Xenopus laevis development
  • Early Development of Xenopus laevis: A Laboratory Manual, Sive, Grainger, and Harland, 2000 [2]
    • comprehensive collection of protocols for the study of early development in Xenopus embryos
  • The Laboratory Xenopus sp., Green, 2010 [3]
    • a highly detailed manual containing Xenopus husbandry, management, veterinary care, and frog and equipment vendor information
  • Xenopus Protocols: Cell Biology and Signal Transduction, Liu (First edition), 2006 [4]
    • step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
  • Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36, Kay and Peng, 1991 [5]
    • detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
  • Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology, Seidman and Soreq, 1997 [6]
    • referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
  • Atlas of Xenopus Development, Bernardini, Prati, Bonetti, and Scari, 1999 [7]
    • scanning, transmission, and light microscopy images of Xenopus embryonic development
  • The early development of Xenopus laevis. An atlas of the histology, Hausen and Riebesell, 1991 [8]
    • detailed histological sections of Xenopus embryonic development
  • Color Atlas of Xenopus laevis Histology, Wiechmann and Wirsig-Wiechmann, 2003 [9]
    • central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis


Select Protocol Sections

Animal Husbandry


Lab Solutions and Reagents (click each to view expanded list)


Generating Embryos


Transgenesis


In Situ Hybridization


Immunohisochemistry


Histology


Other Embryo Staining Protocols


Other Immuno Protocols and Protein Protocols


Nucleic Acid Protocols


Oocyte Transfer Technique (Heasman/Wylie labs)


Xenopus Oocyte and Egg Extracts


Xenopus Tissue Culture


Online Xenopus Resources

External links to Cold Spring Harbor Xenopus Protocols [16] - subscription required, select articles listed

    • Housing and Feeding of Xenopus laevis - Sive et al. [17]
    • Inducing Ovulation in Xenopus laevis - Sive et al. [18]
    • Xenopus laevis In Vitro Fertilization and Natural Mating Methods - Sive et al. [19]
    • Egg Collection and In Vitro Fertilization of the Western Clawed Frog Xenopus tropicalis - Showell & Conlon [20]
    • Isolation of Xenopus Oocytes - Sive et al. [21]
    • Isolating Xenopus laevis Testes - Sive et al. [22]
    • Dejellying Xenopus laevis Embryos - Sive et al. [23]
    • Removing the Vitelline Membrane from Xenopus laevis Embryos - Sive et al. [24]
    • Microinjection of Xenopus Embryos - Sive et al. [25]
    • Defolliculation of Xenopus Oocytes - Sive et al. [26]
    • Microinjection of Xenopus Oocytes - Sive et al. [27]
    • Animal Cap Isolation from Xenopus laevis - Sive et al. [28]
    • Xenopus laevis Keller Explants - Sive et al. [29]
    • Microinjection of RNA and Preparation of Secreted Proteins from Xenopus Oocytes - Sive et al. [30]
    • Calibration of the Injection Volume for Microinjection of Xenopus Oocytes and Embryos - Sive et al. [31]
    • Isolation of DNA from Red Blood Cells in Xenopus - Sive et al. [32]
    • Investigating Morphogenesis in Xenopus Embryos: Imaging Strategies, Processing, and Analysis - Kim & Davidson [33]
    • Low-Magnification Live Imaging of Xenopus Embryos for Cell and Developmental Biology - Wallingford [34]
    • High-Magnification In Vivo Imaging of Xenopus Embryos for Cell and Developmental Biology - Keiserman et al. [35]
    • Preparation of Fixed Xenopus Embryos for Confocal Imaging - Wallingford [36]
    • Whole-Mount Fluorescence Immunocytochemistry on Xenopus Embryos - Lee et al. [37]
    • Generation of Transgenic Xenopus laevis - Kroll & Amaya - [38] [39] [40]
    • In Vivo Time-Lapse Imaging of Neuronal Development in Xenopus - Ruthazer et al. [41]
    • Photoconversion for Tracking the Dynamics of Cell Movement in Xenopus laevis Embryos - Chernet et al. [42]
    • Single-Cell Electroporation in Xenopus - Liu & Haas [43]
    • Imaging Axon Pathfinding in Xenopus In Vivo - Leung & Holt [44]
    • A Versatile Protocol for mRNA Electroporation of Xenopus laevis Embryos - Chernet & Levin [45]


External links to the Journal of Visualized Experiments (JOVE) - Xenopus embryo [46] - excellent video demonstrations

    • Obtaining Eggs from Xenopus laevis Females - Cross & Powers [47]
    • Microinjection of Xenopus Laevis Oocytes - Cohen et al. - [48]
    • Fertilization of Xenopus oocytes, Host Transfer Method - Schneider et al. [49]
    • Tissue Determination Using Animal Cap Transplant (ACT) Assay in X. laevis - Viczian & Zuber [50]
    • Blastomere Explants to Test for Cell Fate Commitment - Grant et al. [51]
    • Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation - Amaya & Kroll [52]
    • Organizer and Animal Pole Explants from X. laevis Embryos and Cell Adhesion Assay - Ogata & Cho [53]
    • Plastic Embedding and Sectioning of X. laevis Embryos - Ogata1 et al. [54]
    • Visualizing RNA Localization in Xenopus Oocytes - Gagnon & Mowry [55]
    • Neural Explant Cultures from Xenopus laevis - Lowery et al. [56]
    • Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling - Itoh & Sokol [57]
    • Dissection, Culture, and Analysis of X. laevis Embryonic Retinal Tissue - McDonough et al. [58]
    • X. tropicalis Egg Extracts to Identify Microtubule-associated RNAs - Sharp & Blower [59]
    • Electroporation of Craniofacial Mesenchyme - Tabler & Liu [60]
    • Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures - Yazejian et al. [61]
    • Single Cell Electroporation in vivo within the Intact Developing Brain - Hewapathirane & Haas - [62]
    • Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells - Joshi & Davidson [63]
    • Preparation and Fractionation of Xenopus laevis Egg Extracts - Cross & Powers [64]
    • In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts - Cross & Powers [65]
    • Study of the DNA Damage Checkpoint using Xenopus Egg Extracts - Willis et al. [66]
    • Two Types of Assays for Detecting Frog Sperm Chemoattraction - Burnett et al. [67]
    • Comparative in vivo Study of gp96 Adjuvanticity in X. laevis - Nedelkovska et al. [68]
    • Patch Clamp and Perfusion Techniques - Yang et al. [69]
    • Patch Clamp Recording of Ion Channels - Brown et al. [70]
    • Cation Transport in Xenopus Oocytes - Dürr et al.[71]
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