Antibodies
Antibody template
12/101 – somites (monoclonal)
3G8- pronephros and otic vesicle (monoclonal)
4A6 – pronephros (monoclonal)
Centrin 1 - basal bodies
phospho-histone H3 - mitotic cells
troponin - heart and other muscle
troponin - cardiac troponin T - tnnt2 - heart
alpha-tubulin - microtubules
gamma-tubulin - Basal bodies, microtubule minus ends
ZO-1
Lectins
ECL - pronephric nephrons
LEL - all pronephric epithelia- also faint lateral line
PNA - cement gland and scattered epidermal cells