Chromosomal FISH-TSA
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Literature:
Krylov V., Macha J., Tlapakova T., Takac M., Jonak J. (2003) The c-SRC1 gene visualized by in situ hybridization on Xenopus laevis chromosomes. Cytogenet. Genome Res. 2003;103(1-2):169-172. [1].
Tlapakova T., Krylov V., Macha J. (2005) Localization, structure and polymorphism of two paralogous Xenopus laevis mitochondrial malate dehydrogenase genes. Chromosome res. 13(7):699-706. [2].
Krylov V., Tlapakova T., Macha J. (2007) Localization of Mdh2 single copy gene on Xenopus tropicalis chromosomes by FISH-TSA technique. Cytogenet. Genome Res. 116:110-112. [3].
Following protocol is designed for the visualization of short cDNA sequences with the minimal lenght of 900-1000 bp.
Protocol: