Phospho-histone H3 - mitotic cells

Antigen: phosporylated histone H3 (Ser10)

Epitope: KLH-conjugated peptide (ARKpSTGGKAPR KQLC) corresponding to amino acids 7-20 of human histone H3

Host Species/Isotype:

Antigen Species: human

Applications: IF, WB

Antibody Name:

Supplier: Upstate, Cat # 06-570 [1]

Comments: not sentitive to bleaching, works post in situ

Contributor: Generated and characterized by Hendzel et al. [2] First use in Xenopus- Saka and Smith 2001 [3]

applications abbreviations: WB - Western blotting, IP - immunoprecipitation, IF - immunofluorescence, IHC - immunohistochemistry, EMSA - electromobility shift assay, ChIP - chromatin immunoprecipitation, FCM - flow cytometry

Additional Comments: Immunoprecipitation/Western blotting:' Yes, very good, 17kD target Optimized conditions: Working dilution approx 1/500 Other species: Pretty much everything from tetrahymena to humans

Anti-phospho-H3 counterstain (red) of an embryo previously stained by FISH (green)