XB-FEAT-948884

zc3hc1

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synonyms and molecular function

from ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPRP Gunkel et al 2021 [1]

"zinc finger protein ZC3HC1 (zinc finger C3HC-type protein 1; [2]) as a genuine NB protein of 53–55 kDa in vertebrates. Formerly, ZC3HC1 had also been called HSCP216, as its cDNA had been among those isolated from hematopoietic stem/progenitor cells [41]. In addition, it was also called ILP1 (inhibitor of apoptosis protein [IAP]-like protein 1), due to some parts of its sequence being similar to that of IAP proteins [42], and NIPA (nuclear interacting partner of ALK), after having isolated it in a yeast two-hybrid screen (Y2H) with a chimeric bait that included the receptor tyrosine kinase ALK [43]. Furthermore, NIPA had been described as a nuclear F-box protein and as primarily existing as a regular part of the SCF-type (SKP1, CUL1, F-box) of multiprotein E3 ubiquitin ligase complexes in the interphase of proliferating cells, while reported to be occurring only in minimal amounts in growth-arrested cells [44,45,46,47,48]. Moreover, NIPA had been described among these studies as targeting cyclin B1 (CCNB1) in interphase, to promote its degradation, and thereby prevent premature mitotic entry due to otherwise increased levels of nuclear CCNB1 earlier in interphase. Here, we show that ZC3HC1/NIPA, which we regard as lacking an F-box and which we neither find to be part of an SCF complex nor required for maintaining the typical subcellular distribution of CCNB1, is an NB-resident protein, with virtually all ZC3HC1 polypeptides located there in certain types of proliferating cells in interphase. Furthermore, we describe ZC3HC1 in vertebrates as occurring at the NEs of all TPR-containing cell types of different morphogenetic origin investigated, including oocytes and non-dividing, terminally differentiated somatic cell types. Moreover, while we show that localisation of ZC3HC1 at the NE depends on TPR polypeptides already present at the NPC, we further demonstrate that also ZC3HC1 itself is needed for enabling additional amounts of TPR to occur bound to the NBs of different human cell types. Finally, we reveal that the ZC3HC1-dependent TPR populations in the somatic cells represent about half the total amount of NE-appended TPR, pointing at the NB’s structure as more complex than formerly anticipated."