Protocols: Difference between revisions
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*'''in situ hybridization''' | *'''in situ hybridization''' | ||
**[[in situ hybridization, brief version (Harland lab)]] | **[[in situ hybridization, brief version (Harland lab)]] | ||
*'''[[fluorescent in-situs and FCIS (Vize lab)]]''' | |||
**[[Wholemount embryo FISH / Double FISH]] | |||
**[[Flourescin Tyramide Synthesis]] | |||
**[[Cy3 Tyramide Synthesis]] | |||
**[[FCIS]] | |||
**[[Chromosomal FISH-TSA]] | |||
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**[[benzidine-peroxidase staining for blood (Thomsen lab)]] | **[[benzidine-peroxidase staining for blood (Thomsen lab)]] | ||
**[[apoptosis via TUNEL (Veenstra lab)]] | **[[apoptosis via TUNEL (Veenstra lab)]] | ||
Revision as of 12:28, 15 November 2011
- Animal Husbandry/Animal Protocols
- in situ hybridization
- Immunohisochemistry
- Other Embryo Staining Protocols
- Xenopus Oocyte and Egg Extracts
- Shechter Lab - crude interphase egg extract ("LSS" - low speed supernatant)
- Shechter Lab - isolation of chromatin from egg extract and histone recovery
- Shechter Lab - preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase)
- Stukenberg Lab - Mike's cycling extracts
- Stukenberg Lab - Sara's cycling extracts
- Stukenberg Lab - Mike's CSF extract
- Stukenberg Lab - Sara's CSF extract
- Stukenberg Lab - assaying H1 kinase in extracts
- Xenopus Tissue Culture
- Purifying Polyclonal Antibodies using Affinity Columns / Chromatography