Protocols

• Animal Husbandry/Animal Protocols
  • inducing egg laying via hCG injection (Vize lab)

• in situ hybridization
  • in situ hybridization, brief version (Harland lab)

• fluorescent in-situs and FCIS (Vize lab)
  • Wholemount embryo FISH / Double FISH
  • Flourescin Tyramide Synthesis
  • Cy3 Tyramide Synthesis
  • FCIS
  • Chromosomal FISH-TSA

• Immunohisochemistry
  • wholemount antibody staining (CSH Protocols) (Wallingford lab)

• Other Embryo Staining Protocols
  • benzidine-peroxidase staining for blood (Thomsen lab)
  • apoptosis via TUNEL (Veenstra lab)

• Oocyte Transfer Technique (Heasman/Wylie labs)
  • Part 1: Choosing Oligodeoxyribonucleotides (ODNs)
  • Part 2: ODN Modification
  • Part 3: Host Transfer Technique
  • Part 4:Fertilization and Development

• Xenopus Oocyte and Egg Extracts
  • Shechter Lab - crude interphase egg extract ("LSS" - low speed supernatant)
  • Shechter Lab - isolation of chromatin from egg extract and histone recovery
  • Shechter Lab - preparation of cycling egg extract (extract that proceeds through multiple rounds of mitosis/interphase)
  • Stukenberg Lab - Mike's cycling extracts
  • Stukenberg Lab - Sara's cycling extracts
  • Stukenberg Lab - Mike's CSF extract
  • Stukenberg Lab - Sara's CSF extract
  • Stukenberg Lab - assaying H1 kinase in extracts

• Xenopus Tissue Culture
  • Stukenberg Lab - tissue culture of Xenopus cell lines S3, XTC
  • Stukenberg Lab - isolation of mitotic chromosomes from XTC cells
  • Stukenberg Lab - in-vitro kinetochore-microtubule binding assays
  • Stukenberg Lab - immunofluorescence with biotinylated antibodies

• Purifying Polyclonal Antibodies using Affinity Columns / Chromatography
  • Making an Affinity Column
  • Running an Affinity Column

• Other Protocols
  • Stukenberg Lab - immunoprecipitation
  • Stukenberg Lab - silver staining protein gels