Protocols: Difference between revisions
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*'''Xenopus Tissue Culture''' | *'''Xenopus Tissue Culture''' | ||
**[[tissue culture of Xenopus cell lines S3, XTC (Stukenberg lab)]] | **[[tissue culture of Xenopus cell lines S3, XTC (Stukenberg lab)]] | ||
**[[ | **[[isolation of mitotic chromosomes from XTC cells (Stukenberg lab)]] | ||
**[[Stukenberg Lab - in-vitro kinetochore-microtubule binding assays]] | **[[Stukenberg Lab - in-vitro kinetochore-microtubule binding assays]] | ||
**[[Stukenberg Lab - immunofluorescence with biotinylated antibodies]] | **[[Stukenberg Lab - immunofluorescence with biotinylated antibodies]] | ||
Revision as of 08:11, 16 November 2011
- Animal Husbandry/Animal Protocols
- In Situ Hybridization
- Immunohisochemistry
- Other Embryo Staining Protocols
- Other Immuno Protocols
- Xenopus Oocyte and Egg Extracts
- crude interphase egg extract (Shechter lab)
- isolation of chromatin from egg extract and histone recovery (Shechter lab)
- preparation of cycling egg extract (Shechter lab)
- cycling extracts, mike's protocol (Stukenberg lab)
- cycling extracts, sara's protocol (Stukenberg lab)
- CSF extract, mike's protocol (Stukenberg lab)
- CSF extract, sara's protocol (Stukenberg lab)
- assaying H1 kinase in extracts (Stukenberg lab)
- Xenopus Tissue Culture
- Purifying Polyclonal Antibodies using Affinity Columns / Chromatography