Protocols: Difference between revisions

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*'''Books for Xenopus Research and Protocols'''
*'''''Books for Xenopus Research and Protocols'''''
**'''Normal Table of Xenopus Laevis (Daudin)''', Nieuwkoop P D, Faber J. (Third edition), 1994 [http://www.xenbase.org/literature/book.do?method=display&bookId=1]
**'''Normal Table of Xenopus Laevis (Daudin)''', Nieuwkoop and Faber (Third edition), 1994 [http://www.xenbase.org/literature/book.do?method=display&bookId=1]
***a systematic and chronological description of Xenopus laevis development
***a systematic and chronological description of Xenopus laevis development
**'''Early Development of Xenopus laevis: A Laboratory Manual''', Sive, Hazel, Grainger, Robert M, Harland, Richard M. 2000 [http://www.xenbase.org/literature/book.do?method=display&bookId=22]
**'''Early Development of Xenopus laevis: A Laboratory Manual''', Sive, Grainger, and Harland, 2000 [http://www.xenbase.org/literature/book.do?method=display&bookId=22]
***comprehensive collection of protocols for the study of early development in Xenopus embryos
***comprehensive collection of protocols for the study of early development in Xenopus embryos
**'''Xenopus Protocols: Cell Biology and Signal Transduction''', Liu X J. (First edition), 2006 [http://www.xenbase.org/literature/book.do?method=display&bookId=206]
**'''Xenopus Protocols: Cell Biology and Signal Transduction''', Liu (First edition), 2006 [http://www.xenbase.org/literature/book.do?method=display&bookId=206]
***step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
***step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
**'''Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36''', Kay B K, Peng H B. 1991 [http://www.xenbase.org/literature/book.do?method=display&bookId=204]
**'''Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36''', Kay and Peng, 1991 [http://www.xenbase.org/literature/book.do?method=display&bookId=204]
***detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
***detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
**'''Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology''', Seidman S, Soreq H. 1997 [http://www.xenbase.org/literature/book.do?method=display&bookId=203]
**'''Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology''', Seidman and Soreq, 1997 [http://www.xenbase.org/literature/book.do?method=display&bookId=203]
***referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
***referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
**'''Atlas of Xenopus Development. ''', Bernardini, G, Prati, M, Bonetti, F, Scari, G, 1999 [http://www.xenbase.org/literature/book.do?method=display&bookId=202]
**'''Atlas of Xenopus Development''', Bernardini, Prati, Bonetti, and Scari, 1999 [http://www.xenbase.org/literature/book.do?method=display&bookId=202]
***scanning, transmission, and light microscopy images of Xenopus embryonic development
***scanning, transmission, and light microscopy images of Xenopus embryonic development
**'''The early development of Xenopus laevis. An atlas of the histology''', Hausen P, Riebesell M. 1991 [http://www.xenbase.org/literature/book.do?method=display&bookId=124]
**'''The early development of Xenopus laevis. An atlas of the histology''', Hausen and Riebesell, 1991 [http://www.xenbase.org/literature/book.do?method=display&bookId=124]
***detailed histological sections of Xenopus embryonic development
***detailed histological sections of Xenopus embryonic development
**'''Color Atlas of Xenopus laevis Histology''', Wiechmann, Allan F , Wirsig-Wiechmann, Celeste R, 2003 [http://www.xenbase.org/literature/book.do?method=display&bookId=82]
**'''Color Atlas of Xenopus laevis Histology''', Wiechmann and Wirsig-Wiechmann, 2003 [http://www.xenbase.org/literature/book.do?method=display&bookId=82]
***central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis
***central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis




*'''Animal Husbandry/Animal Protocols'''
*'''''Animal Husbandry/Animal Protocols'''''
**[[inducing egg laying via hCG injection (Vize lab)]]
**[[inducing egg laying via hCG injection (Vize lab)]]




*'''In Situ Hybridization'''
*'''''In Situ Hybridization'''''
**[[in situ hybridization, brief version (Harland lab)]]
**[[in situ hybridization, brief version (Harland lab)]]
**[[Fluorescent In-Situs and FCIS (Vize lab)]]
**[[Fluorescent In-Situs and FCIS (Vize lab)]]
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*'''Immunohisochemistry'''
*'''''Immunohisochemistry'''''
**[[wholemount antibody staining (CSH Protocols) (Wallingford lab)]]
**[[wholemount antibody staining (CSH Protocols) (Wallingford lab)]]




*'''Other Embryo Staining Protocols'''
*'''''Other Embryo Staining Protocols'''''
**[[benzidine-peroxidase staining for blood (Thomsen lab)]]
**[[benzidine-peroxidase staining for blood (Thomsen lab)]]
**[[apoptosis via TUNEL (Veenstra lab)]]
**[[apoptosis via TUNEL (Veenstra lab)]]
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*'''Other Immuno Protocols'''
*'''''Other Immuno Protocols'''''
**[[immunoprecipitation (Stukenberg lab)]]
**[[immunoprecipitation (Stukenberg lab)]]
**[[silver staining protein gels (Stukenberg lab)]]
**[[silver staining protein gels (Stukenberg lab)]]
Line 50: Line 50:




*'''[[Oocyte Transfer Technique (Heasman/Wylie labs)]]'''
*'''''[[Oocyte Transfer Technique (Heasman/Wylie labs)]]'''''
**[[Part 1: Choosing Oligodeoxyribonucleotides (ODNs)]]
**[[Part 1: Choosing Oligodeoxyribonucleotides (ODNs)]]
**[[Part 2: ODN Modification]]
**[[Part 2: ODN Modification]]
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*'''Xenopus Oocyte and Egg Extracts'''
*'''''Xenopus Oocyte and Egg Extracts'''''
**[[crude interphase egg extract (Shechter lab)]]
**[[crude interphase egg extract (Shechter lab)]]
**[[isolation of chromatin from egg extract and histone recovery (Shechter lab)]]
**[[isolation of chromatin from egg extract and histone recovery (Shechter lab)]]
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*'''Xenopus Tissue Culture'''
*'''''Xenopus Tissue Culture'''''
**[[tissue culture of Xenopus cell lines S3, XTC (Stukenberg lab)]]
**[[tissue culture of Xenopus cell lines S3, XTC (Stukenberg lab)]]
**[[isolation of mitotic chromosomes from XTC cells (Stukenberg lab)]]
**[[isolation of mitotic chromosomes from XTC cells (Stukenberg lab)]]
**[[in-vitro kinetochore-microtubule binding assays (Stukenberg lab)]]
**[[in-vitro kinetochore-microtubule binding assays (Stukenberg lab)]]

Revision as of 16:05, 16 November 2011

  • Books for Xenopus Research and Protocols
    • Normal Table of Xenopus Laevis (Daudin), Nieuwkoop and Faber (Third edition), 1994 [1]
      • a systematic and chronological description of Xenopus laevis development
    • Early Development of Xenopus laevis: A Laboratory Manual, Sive, Grainger, and Harland, 2000 [2]
      • comprehensive collection of protocols for the study of early development in Xenopus embryos
    • Xenopus Protocols: Cell Biology and Signal Transduction, Liu (First edition), 2006 [3]
      • step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents
    • Xenopus laevis: Practical Uses in Cell and Molecular Biology, Volume 36, Kay and Peng, 1991 [4]
      • detailed description of a wide range of uses for Xenopus laevis oocytes and embryos in cell and molecular biology
    • Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology, Seidman and Soreq, 1997 [5]
      • referenced guide to the use of microinjected embryos studying the role and regulation of nervous system proteins during development
    • Atlas of Xenopus Development, Bernardini, Prati, Bonetti, and Scari, 1999 [6]
      • scanning, transmission, and light microscopy images of Xenopus embryonic development
    • The early development of Xenopus laevis. An atlas of the histology, Hausen and Riebesell, 1991 [7]
      • detailed histological sections of Xenopus embryonic development
    • Color Atlas of Xenopus laevis Histology, Wiechmann and Wirsig-Wiechmann, 2003 [8]
      • central source on the microscopic anatomy of cells, tissues, and major organs of Xenopus laevis









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