Antibodies and lectins

Antibodies

Antibody template

12/101 - somites, skeletal muscle marker

3G8- pronephros and otic vesicle

4A6 – pronephros

zn-12 - neuronal marker

CBFβ - cbfb - blood, CNS, blood vessels

centrin 1 - cetn1 - basal bodies

collagen type II - col2a1 - ECM

collagen type II - col2a1 - ECM broad

eya1 - eya1 - developing ear

fibrillin-2 - fbn2 - secreted from endothelium

flk1 - VEGFR2 - kdr - endothelium, blood

phospho-histone H3 - mitotic cells

hnk-1 - b3gat1 - migrating neural crest

LMO2 - lmo2 - blood, presomitic mesoderm, CNS

slug - snai2 - endothelial to mesenchyme transition, neural crest

phospho-smad 1/5/8 - smad1 - smad8

troponin - heart and other muscle

troponin - cardiac troponin I - tnni3 - heart

troponin - cardiac troponin T - tnnt2 - heart

alpha-tubulin - tuba4b - microtubules

acetylated alpha-tubulin - tuba4b

gamma-tubulin - tubg1 - basal bodies, microtubule minus ends

VEGFA - vegfa - somites, lateral plate mesoderm, head, hypochord

ZO-1 - tjp1

Lectins

ECL - pronephric nephrons

LEL - all pronephric epithelia- also faint lateral line

PNA - cement gland and scattered epidermal cells