Antibodies and lectins
Antibodies
Monoclonal 3G8- pronephros and otic vesicle
Polyclonal anti-phospho-H3 - mitotic cells
Alpha-tubulin - For labeling microtubules
Gamma-tubulin - Basal bodies, microtubule minus ends
Lectins
ECL - stains pronephric nephrons
LEL - all pronephric epithelia- also faint lateral line